Genomic and Immunological Identification of Canine Distemper Virus (CDV) and Investigation of Coinfection with Bordetella bronchiseptica Among Dogs in Iran

Background: Canine distemper virus (CDV) is an extremely contagious pathogen that causes deadly diseases in carnivores worldwide. Objectives: Considering the effect of CDV on host immune system and increased risk other infections, present study aimed to investigate incidence coinfection with Bordetella bronchiseptica, using genomic serological methods. Methods: In this study, 50 blood samples, eye respiratory swabs, gastrointestinal tract swabs were taken from dogs, which showed symptoms involvement, suggesting infection. Also, seemingly healthy dogs included study. The animals referred Isfahan clinics between spring 2018 winter 2019. For initial diagnosis by immunological methods, a rapid immunochromatography kit was used. To for genomes both pathogens, after DNA RNA extraction reverse transcription extracted PCR assay performed specific primers. Results: Based results RT-PCR assay, samples suspected infection, 37 positive presence nucleic acids, 20 B. bronchiseptica acids. three CDV, 15 Conclusions: 100 infection apparently (12 dogs). However, no significant relationship found infections. It seems future studies larger sample size can provide us more accurate results.